SOLVED: A protein with molecular weight 10 kDa can form two structural isoforms of A and B in solution The two formations can easily interconvert to each other in solution. Protein with the two states

BIOCHEM:
Using the Stock A (1.0 mg/ml) to prepare 1 ml of 8.0 µg/ml, 1 ml of
10.0 µg/ml, and 1 ml of 12.0 µg/ml of solutions. Read the
absorbance of each solution at the wavelength of 600 nm (A600).
Perform the determination in duplicate. Plot A600 versus
concentration using the average of the measured absorbance values
from the two separate determinations. Calculate the extinction
coefficient as the following:
Extinction coefficient = Absorbance /
(Concentration X Path length)
where the concentration is in mg/ml and the path length is in
cm. What dilutions of the stock are each of the
prepared solutions (i.e., 1/x)? The molecular weight
of A is 290 g/mole. Re-calculate the extinction
coefficient with the concentration in mM. Note that
the newly calculated extinction coefficient will contain a
mM-1 term.
Path length “L” = 1cm
8.0µL of stock A with .992 µL of
H2O the experimental
absorbance was recorded to be 0.254
10.0µL of stock A with .990 µL of H2O the
experimental absorbance was recorded to be 0.414
12.0µL of stock A with .988 µL of H2O the experimental
absorbance was recorded to be 0.64